Visualize and Quantify | Assess Biodistribution and Function | Products & Services | Resources
Visualize and Quantify Small RNA Delivery and Function
Synthetic oligonucleotides like small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs) function as a form of gene therapy by precisely targeting genes to control their expression. With RNAscope ISH technology you can visualize small regulatory RNAs (smRNAs) alone, or along with its mRNA targets. Assess oligonucleotide therapy delivery, biodistribution, and interrogate smRNA biomarkers along with cell specific/morphology/functional biomarkers.
Unlock Answers to Critical Questions
Visualize Synthetic and Endogenous RNA to Advance Your Small RNA Therapeutic
- Assess in vivo biodistribution of the ASOs or small RNA and determine cellular uptake, and persistence over time.
- Visualize 1 small RNA (ASO, miRNA, siRNA) + up to 3 mRNA targets in intact tissues.
- Evaluate expression of the target gene and cell-specific marker genes with morphological context in heterogeneous tissues.
- Safety and efficacy screen for potential toxicity and off-target effects.
miRNAscope™ Assays
miRNAscope™ Assays
The miRNAscope assay allows visualization of ASOs, miRNA, siRNA, and other nucleic acid targets between 17-50 nucleotides.
RNAscope PLUS Assays
RNAscope PLUS Assays
The RNAscope™ Plus smRNA-RNA Assay enables visualization of one small RNA (ASO, miRNA, siRNA) plus up to three mRNA markers.
miRNAscope Assay
The targeting siRNA was detected using the siRNA probe in the siRNA-treated mouse liver tissue samples and not in the vehicle tissue samples using the miRNAscope assay, indicating successful delivery of the siRNA-based therapy. Expression of target mRNA was detected using the RNAscope assay and appeared significantly reduced in the siRNA-treated tissue compared to vehicle-treated tissue suggesting successful knock-down of the target mRNA expression with the siRNA-based therapy.
RNAscope™ Plus small RNA-RNA Assay
Detection of Small RNAs and Target RNAs. The RNAscope Plus assay leverages the patented core technology that enables signal amplification and background suppression for multiplex fluorescent visualization of one small RNA with up to three mRNAs in formalin-fixed paraffin-embedded (FFPE) and fresh frozen samples.
On Demand Webinar
Multiomics spatial biodistribution visualization tool for RNAi and oligonucleotide-based therapies
RNAscope Professional Assay Services
Our Professional Assay Services offer you direct access to the developers of RNAscope in situ hybridization (ISH) technology. Accelerate your journey in target validation, preclinical, and clinical development with the promise that we will provide consistent and trustworthy data to drive your projects forward.
RNAscope ISH Technology - Product Formats
Multiomic Spatial Solutions for RNA and Protein Co-detection
RNAscope~20 ZZ probe pairs Designed to > 300 nt.
| BaseScope™1-3 ZZ probe pairs Designed to ~ 50-300 nt.
| miRNAscope™Designed to ~17-50 nt.
| RNAscope PlusRNA species of varied sizes.
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Related Resources
Reference
- S12 Nonclinical Biodistribution Considerations for Gene Therapy Products; Guidance for Industry; U.S. Department of Health and Human Services Food and Drug Administration; Center for Drug Evaluation and Research (CDER); Center for Biologics Evaluation and Research (CBER) May 2023; ICH-Safety.