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Advanced Cell Diagnostics, a Bio-Techne brand offers multiple, unique options for RNA chromogenic in situ hybridization assays (RNA CISH). Two types of detection chemistries are available depending on your research need, preferred manual or automated workflow, and your preferred imaging platform.
Unprecedented Sensitivity and Specificity
Unprecedented Sensitivity and Specificity
- RNAscope assays allow single RNA molecule detection with our ACD patented Z probe pair design
- Rigorous double Z probes design eliminates cross-hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology
Universal
Universal
- Our RNAscope ISH assays work for virtually ANY gene in ANY species in ANY tissue
Flexible Workflows
Flexible Workflows
- Assay options for manual use or increasing throughput of sample analysis using automated platforms or manual use
- Two chemistry options to fit needs
RNAscope Single-plex Chromogenic Assays
- RNAscope 2.5 HD Manual assay enables single target detection with bright field microscopy using either Diaminobenzidine (DAB) or Fast Red dyes
- RNAscope VS Universal HRP and RNAscope VS Universal AP assays are our automated solutions enabling customers with high throughput single RNA detection on the Roche DISCOVERY ULTRA
- RNAscope 2.5 LS Brown, RNAscope 2.5 LS Red, RNAscope 2.5 LSx Brown or RNAscope 2.5 LSx Red assays are our automated solution which enables customers with high throughput detection of RNA targets using the Leica BOND RX research staining platform
RNAscope Duplex Chromogenic Assays
Our RNAscope Duplex assays are designed for simultaneous in situ detection of two RNA species using probe channels C1 and C2. Common applications include co-localization studies to map co-expression of two targets within the same cellular context (e.g. secreted ligand and its receptor) or to profile gene expression in a specific cell type expressing a known marker (e.g. a specific stem cell marker).
- RNAscope™ 2.5 HD Duplex Assay – enables manual detection of two RNA targets using HRP-based Green and AP-based Fast Red chromogens
- RNAscope™ VS Duplex Assay – enables high throughput detection of two RNA targets on the
- RNAscope™ 2.5 LS Duplex Assay offers 12-plex RNA detection in FFPE tissues and up to 48-plex RNA detection in frozen tissues with use of HiPlex Up.
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