H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Integrin alpha8 in H4 Human Cell Line.

Integrin a8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin alpha8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: H4 human neuroglioma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed H4 human neuroglioma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta1. Integrin alpha8/ beta1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta1 and Nephronectin. It is principally expressed in smooth muscle cells. Human alpha8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha8 shares 90% aa sequence identity with mouse alpha8 protein.

Alternate Names

ITGA8

Entrez Gene IDs

8516 (Human); 241226 (Mouse); 84381 (Rat)

Gene Symbol

ITGA8

UniProt

P53708

Additional Integrin alpha 8 Products

Product Documents for Human Integrin alpha8 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human Integrin alpha8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6194

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha 8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha8 Antibody

Detection of Integrin alpha8 in H4 Human Cell Line by Flow Cytometry.