Detects human IGF-I R/IGF1R in sandwich ELISAs and Western blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse IGF-I R/IGF1R Antibody

Detection of Human IGF-I R/IGF1R by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.

Detection of IGF-I R/IGF1R in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

IGF-I R/IGF1R in MCF‑7 and HDLM Human Cell Lines.

IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane in MCF-7 cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human/Mouse IGF-I R/IGF1R Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: MCF-7 human breast cancer cell line

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse heart

Western Blot

1 µg/mL
Sample: NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.

Human IGF-I R/IGF1R Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IGF-I R/IGF1R Biotinylated Antibody (Catalog # BAF391)
Standard: Recombinant Human IGF-I R/IGF1R Protein, CF (Catalog # 391-GR)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB391 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Human/Mouse IGF-I R/IGF1R Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse IGF-I R/IGF1R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse IGF-I R/IGF1R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB391

Key Product Details

Species Reactivity

Validated:

Cited:

Applications