Human/Mouse IkB-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4299
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IkB-alpha
Met1-Leu314
Accession # Q9Z1E3
Met1-Leu314
Accession # Q9Z1E3
Specificity
Detects human and mouse IkB-alpha in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse IkB-alpha Antibody
Detection of Human/Mouse IkB-alpha by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, LNCaP human prostate cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse IkB-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for IkB-a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IkB‑ alpha in HeLa Human Cell Line.
IkB-a was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse IkB-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IkB‑ alpha in HeLa Human Cell Line.
IkB-a was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse IkB-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse IkB-alpha Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and RAW 264.7 Mouse Monocyte/Macrophage Cell Line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and RAW 264.7 Mouse Monocyte/Macrophage Cell Line
Knockout Validated
IkB‑ alpha
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
IkB‑ alpha knockout HEK293T cell line.
Western Blot
0.1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, LNCaP human prostate cancer cell line, and Daudi human Burkitt's lymphoma cell line
Sample: NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, LNCaP human prostate cancer cell line, and Daudi human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-alpha
NF kappaB inhibitor alpha (IkB-alpha) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-alpha degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Gene Symbol
NFKBIA
UniProt
Additional IkB-alpha Products
Product Documents for Human/Mouse IkB-alpha Antibody
Product Specific Notices for Human/Mouse IkB-alpha Antibody
For research use only
Loading...
Loading...
Loading...
Loading...