Human PDGF R beta Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # AF385
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey), Rabbit
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
Leu33-Phe530 (Glu241Asp)
Accession # P09619
Leu33-Phe530 (Glu241Asp)
Accession # P09619
Specificity
Detects human PDGF R beta in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human PDGF R beta Antibody
PDGF R beta in Human Breast Cancer Tissue.
PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody.
Recombinant Human PDGF R beta Fc Chimera (385-PR) inhibits Recombinant Human PDGF-BB (220-BB) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND50 is typically 10-40 µg/mL.
Detection of PDGF R beta in U87MG cells by Flow Cytometry.
U87MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Applications for Human PDGF R beta Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: BUD‑8 human fibroblast cell line, U87MG and U118MG
Sample: BUD‑8 human fibroblast cell line, U87MG and U118MG
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
2 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line and U2OS human osteosarcoma cell line
Sample: SH-SY5Y human neuroblastoma cell line and U2OS human osteosarcoma cell line
Neutralization
Measured by its ability to neutralize PDGF R beta inhibition of PDGF-BB-dependent proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 2 μg/mL Recombinant Human PDGF R beta Fc Chimera and 4 ng/mL Recombinant Human PDGF-BB.
Reviewed Applications
Read 3 reviews rated 4 using AF385 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R beta
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.
References
- Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
Long Name
Platelet-derived Growth Factor Receptor beta
Alternate Names
CD140b, PDGFRB
Gene Symbol
PDGFRB
UniProt
Additional PDGF R beta Products
Product Documents for Human PDGF R beta Antibody
Product Specific Notices for Human PDGF R beta Antibody
For research use only
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