IkB-alpha Antibody (6A920) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56507

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-56507
NB100-56507SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A920

Format

BSA Free

Concentration

1.0 mg/ml

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

Partial recombinant protein corresponding to amino acid residues 32-291 of human IkB-alpha.

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IkB-alpha Antibody (6A920) - BSA Free

Knockout Validated: IkB-alpha Antibody (6A920) - BSA Free [NB100-56507]

Knockout Validated: IkB-alpha Antibody (6A920) - BSA Free [NB100-56507]

Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Western Blot: IkB-alpha Antibody (6A920)BSA Free [NB100-56507]

Western Blot: IkB-alpha Antibody (6A920)BSA Free [NB100-56507]

Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG.
Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) - BSA Free [NB100-56507]

Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) - BSA Free [NB100-56507]

Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) [NB100-56507] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
View 7 more images

Applications for IkB-alpha Antibody (6A920) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.25-1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1 ug/ml

Simple Western

1:20

Western Blot

1-2 ug/ml
Application Notes
A 40 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

IkBa Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses IkBa becomes phosphorylated, promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Entrez Gene IDs

4792 (Human); 18035 (Mouse)

Gene Symbol

NFKBIA

UniProt

P25963

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody (6A920) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IkB-alpha Antibody (6A920) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.