IkB-alpha Antibody (6A920) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80787

Novus Biologicals, part of Bio-Techne
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NBP2-80787
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Catalog #
BSA Free
NB100-56507
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A920

Format

Azide and BSA Free

Concentration

1 mg/ml

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IkB-alpha Antibody (6A920) - Azide and BSA Free

Western Blot: IkB-alpha Antibody (6A920)Azide and BSA Free [NBP2-80787]

Western Blot: IkB-alpha Antibody (6A920)Azide and BSA Free [NBP2-80787]

Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787]

Immunocytochemistry/ Immunofluorescence: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787]

Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787]

Immunohistochemistry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787]

Immunohistochemistry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min
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Applications for IkB-alpha Antibody (6A920) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.25-1 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:20-1:1000

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1 ug/ml

Simple Western

1:20

Western Blot

1-2 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

IkBa Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses IkBa becomes phosphorylated, promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody (6A920) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IkB-alpha Antibody (6A920) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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