IkB-alpha Antibody (6A920) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24815

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-56507AF350

Alexa Fluor 405

NB100-56507AF405

Alexa Fluor 488

NB100-56507AF488

Alexa Fluor 532

NB100-56507AF532

Alexa Fluor 594

NB100-56507AF594

Alexa Fluor 647

NB100-56507AF647

Alexa Fluor 700

NB100-56507AF700

Alexa Fluor 750

NB100-56507AF750

Allophycocyanin

NB100-56507APC

Allophycocyanin/Cy7

NB100-56507APCCY7

Biotin

NB100-56507B

DyLight 350

NB100-56507UV

DyLight 405

NB100-56507V

DyLight 488

NB100-56507G

DyLight 550

NB100-56507R

DyLight 594

NB100-56507DL594

DyLight 650

NB100-56507C

DyLight 680

NB100-56507FR

DyLight 755

NB100-56507IR

FITC

NB100-56507F

HRP

NB100-56507H

Janelia Fluor 549

NB100-56507JF549

Janelia Fluor 646

NB100-56507JF646

mFluor Violet 450 SE

NB100-56507MFV450

mFluor Violet 500 SE

NB100-56507MFV500

mFluor Violet 610 SE

NB100-56507MFV610

PE

NB100-56507PE

PE/Atto594

NB100-56507PEATT594

PE/Cy5.5

NB100-56507PECY55

PE/Cy7

NB100-56507PECY7

PerCP

NB100-56507PCP

Forumulation

Catalog #

BSA Free

NB100-56507

Azide and BSA Free

NBP2-80787

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockout Validated

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 6A920

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Specificity

A 40 kDa band is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Applications for IkB-alpha Antibody (6A920) [Biotin]

Application

Recommended Usage

ELISA

1:100-1:2000

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha Antibody (6A920) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for IkB-alpha Antibody (6A920) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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