Human/Mouse TIMP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF971
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, Immunohistochemistry, Knockout Validated, Neutralization, Western Blot
Cited:
Functional Assay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP-2
Cys27-Pro220
Accession # P16035
Cys27-Pro220
Accession # P16035
Specificity
Detects human and mouse TIMP‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human TIMP-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Mouse TIMP-2 Antibody
Detection of Human TIMP‑2 by Western Blot.
Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TIMP-2 in Human Ovarian Cancer Tissue.
TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human TIMP-2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human TIMP-2 knockout HeLa human cervical epithelial carcinoma cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human/Mouse TIMP-2 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and normal human ovarian array
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and normal human ovarian array
Knockout Validated
TIMP‑2
is specifically detected in HeLa human cervical epithelial carcinoma parental
cell line but is not detectable in TIMP‑2 knockout HeLa human
cervical epithelial carcinoma cell line.
Western Blot
1 µg/mL
Sample: Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023), HeLa human cervical epithelial carcinoma cell line and human placenta tissue
Sample: Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023), HeLa human cervical epithelial carcinoma cell line and human placenta tissue
Neutralization
Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL, Catalog # 971-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MPN or 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 2.6 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4 using AF971 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-2
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
UniProt
Additional TIMP-2 Products
Product Documents for Human/Mouse TIMP-2 Antibody
Product Specific Notices for Human/Mouse TIMP-2 Antibody
For research use only
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