Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel

For the identification and characterization of neural progenitor cells.

Why use a Panel of Antibodies to Identify and Characterize Neural Progenitor Cells?

Neural progenitor cells (NPC) reside in several areas of the embryonic and adult central nervous system and are defined by their ability to self-renew and differentiate into neurons, astrocytes, and oligodendrocytes. Various molecular markers can be used to identify NPC populations in vivo and in vitro.However, since most markers are expressed in a variety of cell types, several markers must be examined to thoroughly define a cell population of interest.

The Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel is designed for the characterization of human/mouse/rat NPC marker expression by immunocytochemistry (all markers) and flow cytometry (CXCR4 and SSEA-1). The panel can be used to detect the following marker molecules: CXCR4, Musashi-1, Nestin, Notch-1, SOX1, SOX2, SSEA-1, and Vimentin.

The Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel:

• Includes eight antibodies to increase confidence in cell identification and characterization.
• Compatible with detection of SSEA-1 and CXCR4 by flow cytometry, which can be completed in less than two hours.
• Compatible with detection of all markers by immunocytochemistry, which can be completed in less than six hours.

Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel (Catalog # SC025) includes eight antibodies that can be used to detect expression of CXCR4, Musashi, Nestin, Notch-1, SOX1, SOX2, SSEA-1, and Vimentin.

• Mouse Anti-CXCR4 Monoclonal Antibody (clone 44708, isotype mouse IgG_2A)
• Goat Anti-Musashi-1 Antigen Affinity-purified Polyclonal Antibody
• Mouse Anti-Nestin Monoclonal Antibody (clone 307501, isotype mouse IgG_2A)
• Goat Anti-Notch-1 Antigen Affinity-purified Polyclonal Antibody
• Goat Anti-SOX1 Antigen Affinity-purified Polyclonal Antibody
• Goat Anti-SOX2 Antigen Affinity-purified Polyclonal Antibody
• Mouse Anti-SSEA-1 Monoclonal Antibody (clone MC-480, isotype mouse IgM)
• Rat Anti-Vimentin Monoclonal Antibody (clone 280618, isotype rat IgG_2A)

Each antibody is supplied as a 10X stock; enough for 25 assays when used in a 50 µL staining volume per assay.

Stability and Storage

The unopened kit can be stored at 2 °C to 8 °C and should be used within one year of receipt.

The opened/Reconstituted Reagents may be stored for up to 1 month at 2 °C to 8 °C or aliquoted and stored at =-20 °C in a manual defrost freezer for up to 6 months. Avoid repeated freeze-thaw cycles.

PRECAUTION

Safe laboratory procedures should be followed and protective clothing should be worn when handling kit reagents.

LIMITATIONS OF THE PROCEDURE

• FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses have not been established.

Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.

To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.