Proteome Profiler Human Angiogenesis Array Kit Best Seller

A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.

Troubleshooting Guide

Principle of the Assay

The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.

Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Antibody Detection Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 ^°C to 8 ^°C. Do not use past kit expiration date.

Scientific Data Examples for Proteome Profiler Human Angiogenesis Array Kit

	Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software.
	The Human Angiogenesis Array detects multiple analytes in cell culture supernates. A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
	The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma. Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.