Proteome Profiler Mouse Cytokine Array Kit, Panel A Best Seller
R&D Systems, part of Bio-Techne | Catalog # ARY006
Key Product Details
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The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane. |
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The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
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The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |