Proteome Profiler Human Apoptosis Array Kit Best Seller

A membrane-based antibody array for the parallel determination of the relative levels of human apoptosis-related proteins. Validated for analyte detection in cell lysates.

Troubleshooting Guide

Principle of the Assay

The Proteome Profiler Human Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

• 4 Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Lysis Buffer
• Wash Buffer
• Antibody Detection Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 ^°C to 8 ^°C. Do not use past kit expiration date.

Assays for analytes represented in the Human Apoptosis Array Kit

Western blotting antibodies for analytes related to the Human Apoptosis Array Kit

Scientific Data Examples for Proteome Profiler Human Apoptosis Array Kit

	Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. Figure 1A: K562 human chronic myelogenous leukemia cells. Figure 1B: THP-1 human acute monocytic leukemia cells. Figure 1C: SK-Mel-28 human malignant melanoma cells.
	Determination of Relative Levels of Human Apoptosis-related Proteins Using Proteome Profiler Human Apoptosis Array Kit. Jurkat human acute T cell leukemia cells were either untreated or treated with 25 µM etoposide for 6 hours. Etoposide is a topoisomerase II inhibitor that causes caspase-3 cleavage as apoptosis is induced.