Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected angiogenesis-related proteins. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma and saliva.
Troubleshooting Guide
Key Benefits
- Detects 55 human angiogenesis-related proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are rquired. Refer to
protocol.
Principle of the Assay
The Proteome Profiler Human Angiogenesis Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of bound analyte. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules.
Why Use an Antibody Array to Detect Angiogenesis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample. |
| Activin A | FGF-7/KGF | PD-ECGF |
| ADAMTS-1 | GDNF | PDGF-AA |
| Angiogenin | GM-CSF | PDGF-AB/PDGF-BB |
| Angiopoietin-1 | HB-EGF | Persephin |
| Angiopoietin-2 | HGF | CXCL4/PF4 |
| Angiostatin/Plasminogen | IGFBP-1 | PlGF |
| Amphiregulin | IGFBP-2 | Prolactin |
| Artemin | IGFBP-3 | Serpin B5/Maspin |
| Tissue Factor/Factor III | IL-1 beta | Serpin E1/PAI-1 |
| CXCL16 | CXCL8/IL-8 | Serpin F1/PEDF |
| DPPIV/CD26 | LAP (TGF-beta 1) | TIMP-1 |
| EGF | Leptin | TIMP-4 |
| EG-VEGF | CCL2/MCP-1 | Thrombospondin-1 |
| Endoglin/CD105 | CCL3/MIP-1 alpha | Thrombospondin-2 |
| Endostatin/Collagen XVIII | MMP-8 | uPA |
| Endothelin-1 | MMP-9 | Vasohibin |
| FGF acidic | NRG1-beta 1 | VEGF |
| FGF basic | Pentraxin 3 | VEGF-C |
| FGF-4 | | |
Assays for analytes represented in the Human Angiogenesis Antibody Array Kit
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
